• 说明书丨HRH44911-Oxamic acid sodium.pdf

产品信息:

产品描述：

    Oxamic acid (oxamate) sodium salt 是一种 LDH-A (乳酸脱氢酶-A) 抑制剂。Oxamic acid sodium salt 表现出抗肿瘤活性，对癌细胞的抗增殖活性，并可诱导细胞凋亡。

体外研究：

Oxamic acid 抑制 A2780 和 SKOV3 细胞的增殖、迁移和侵袭。

Oxamic acid (10 μM；24-72 h) 在剂量和时间上抑制细胞增殖两种 NPC 癌细胞中的依赖性方式。

Oxamic acid sodium (0-100 mM；24 小时) 在 CNE-1 和 CNE 中诱导 G2/M 期细胞周期停滞-2 细胞。

Oxamic acid sodium (0-100 mM；48 小时) 通过 caspase-3 激活和线粒体途径诱导 NPC 细胞凋亡。

Oxamic acid sodium (0-100 mM；24 小时) 增加 NPC 细胞中的 ROS 水平。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay：

Cell Line：CNE-1 and CNE-2 cells

Concentration：10 μM

Incubation Time：24-72 hours

Result：Showed IC50s of 74.6, 32.4 and 17.8 mM and 62.3, 44.5, 31.6 mM at 24, 48 and 72 h in the CNE-1 and CNE-2 cancer cells, respectively.

Apoptosis Analysis：

Cell Line：NPC cells

Concentration：0, 20, 50 and 100 mM

Incubation Time：48 hours

Result：Showed the increasement of early and late apoptotic cells in a dose-dependent manner.

Increased the expression of pro-apoptotic Bax and cleaved-caspase-3, while reduced the anti-apoptotic signals of Bcl-2 and pro-caspase-3.

Cell Cycle Analysis：

Cell Line：CNE-1 and CNE-2 cells

Concentration：0, 20, 50 and 100 mM

Incubation Time：24 hours

Result：Showed a dose-dependent increase in the numbers of CNE-1 and CNE-2 cells in the G2/M phase.

体内研究：

Oxamic acid sodium (腹腔注射；750 mg/kg；每日一次；3 w) 联合放射处理可提高体内肿瘤抑制效果。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model：Female Balb/c nude mice injected with CNE-1 cells

Dosage：750 mg/kg

Administration:Intraperitoneal injection; 750 mg/kg; once daily; 3 weeks

Result：Inhibited the tumor growth when compared to either oxamate alone or irradiation alone.

产品属性：

储存方法：

4°C, sealed storage, away from moisture

In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)