价格:2,770
货号Anti-Renilla Luciferase pAb
产品详情
产品详情
产品信息
产品名称 |
产品编号 |
规格 |
Anti-Renilla Luciferase pAb |
PM047 |
100 µL |
产品描述
Renilla Luciferase is a 36 kDa luminescent enzyme isolated from Sea Pansy (Renillareniformis). The enzyme catalyzes coelenterate-luciferin (oelenterazine) oxidation to produce light. Renilla Luciferase is frequently used as a reporter protein to quantify promoter activities and expression levels in transgenic cells.
名称 |
内容 |
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来源(Source) |
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with full-length of Renilla Luciferase protein (1-311 aa). |
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产品形态(Formulation) |
100µL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained |
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储存(Storage) |
This antibody solution is stable for one year from the date of purchase when stored at -20°C |
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反应(Reactivity) |
This antibody reacts with Renilla Luciferase (36 kDa) on Western blotting, Immunoprecipitation and Immunocytochemistry. It does not cross react with firefly Luciferase |
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应用验证(Applications) |
Western blotting |
1:1,000 |
Immunoprecipitation |
2µL/250µL of cell extract from 5 x 106 cells |
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Immunohistochemistry |
not recommended |
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Immunocytochemistry |
1:100-1:200 |
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Immunocytochemistry (paraffin section) |
1:1,000 Heat treatment is necessary for paraffin embedded sections. Microwave oven; twice for 10 minutes each in 10 mM citrate buffer (pH 6.3) |
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Flow cytometry |
Not tested |
Detailed procedure is provided in the following
注意事项
本产品仅作科研用途!