Anti-Renilla Luciferase pAb

Anti-Renilla Luciferase pAb

价格:2,770

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产品详情

产品信息

 

产品名称

产品编号

规格

Anti-Renilla Luciferase pAb

PM047

100 µL

 

产品描述

 Renilla Luciferase is a 36 kDa luminescent enzyme isolated from Sea Pansy (Renillareniformis). The enzyme catalyzes coelenterate-luciferin (oelenterazine) oxidation to produce light. Renilla Luciferase is frequently used as a reporter protein to quantify promoter activities and expression levels in transgenic cells.

 

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内容

来源(Source)

This antibody was purified from rabbit serum

using affinity column. The rabbit was immunized with

full-length of Renilla Luciferase protein (1-311 aa).

产品形态(Formulation)

100µL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained

储存(Storage)

This antibody solution is stable for one year from the date of purchase when stored at -20°C

反应(Reactivity)

This antibody reacts with Renilla Luciferase (36 kDa) on Western blotting, Immunoprecipitation and Immunocytochemistry. It does not cross react with firefly Luciferase

应用验证(Applications)

Western blotting

1:1,000

Immunoprecipitation

2µL/250µL of cell extract from 5 x 106 cells

Immunohistochemistry

 not recommended

Immunocytochemistry

1:100-1:200

Immunocytochemistry (paraffin section)

1:1,000

Heat treatment is necessary for paraffin embedded sections. Microwave oven; twice for 10 minutes each in 10 mM citrate buffer (pH 6.3)

Flow cytometry

Not tested

Detailed procedure is provided in the following

注意事项

本产品仅作科研用途!

 

 

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