价格:3,430
货号Anti-T7-tag pAb
产品详情
产品详情
产品信息
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产品名称 |
产品编号 |
规格 |
价格 |
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Anti-T7-tag pAb |
PM022 |
100μL |
3430.00 |
产品描述
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tagantibodies can serve as universal purification or detection reagents for any tag-containing protein. Anti-T7-tag antibody is directed against the 11 amino acid of gene 10 leader peptide (MASMTGGQQMG) expressed by many translation vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage. Anti-T7-tag antibody is a useful reagent to easily identify, detect, or purify of T7-tag fusion proteins from cell lysates.
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名称 |
内容 |
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来源(Source) |
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with carrier protein (CP) conjugated synthetic peptide, CP-MASMTGGQQMG. |
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反应(Reactivity) |
This antibody reacts with T7-tag on Western blotting and Immunoprecipitation. |
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产品形态(Formulation) |
100 μL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
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存储(Storage) |
This antibody is stable for one year from the date of purchase when stored at -20°C. |
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应用验证(Applications-Confiirmed) |
Western blotting |
1:1,000 |
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Immunoprecipitation |
5 µL/sample |
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Immunohistochemistry |
Not tested |
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Immunocytochemistry |
Not tested |
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Flow cytometry |
Not tested |
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Chromatin Immunoprecipitation |
Not tested* |
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*It is reported that this polyclonal antibody can be used in Chromatin Immunoprecipitation in the following.
注意事项
本产品仅作科研用途！