价格:3,430
货号Anti-S-tag pAb
产品详情
产品详情
产品信息
产品名称 |
产品编号 |
规格 |
价格 |
Anti-S-tag pAb |
PM021 |
100μL |
3430.00 |
产品描述
Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. Short sequences encoding the epitope tag are cloned in-frame with target DNA to produce fusion proteins containing the epitope tag peptide. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Anti-epitope tag antibodies can serve as universal purification or detection reagents for any tag-containing protein. S-tag is an epitope tag composed of a 15 residues peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. S-tag can combine with S-protein to form functional RNase S, allowing detection and quantification of S-tag fusion proteins by enzymatic assays. However, antibody against the S-tag sequence provides a more convenient and flexible method to easily identify, detect, or purify S-tag containing fusion proteins.
名称 |
内容 |
|
来源(Source) |
This antibody was purified from rabbit serum using affinity column. The rabbit was immunized with carrier protein (CP) conjugated synthetic peptide, CP-KETAAAKFERQHIDS. |
|
反应(Reactivity) |
This antibody reacts with S-tagged protein on Western blotting and Immunoprecipitation. |
|
产品形态(Formulation) |
100 µL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. |
|
存储(Storage) |
This antibody is stable for one year from the date of purchase when stored at -20°C. |
|
应用验证(Applications-Confiirmed) |
Western blotting |
1:1,000 for a chemiluminescence detection system |
Immunoprecipitation |
5 µL/sample |
|
Immunohistochemistry |
Not tested |
|
Immunocytochemistry |
Not tested |
|
Flow cytometry |
Not tested |
注意事项
本产品仅作科研用途!